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| Registros recuperados: 56 | |
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| Alain, Karine; Querellou, Joel. |
| Since the invention of the Petri dish, there have been continuous efforts to improve efficiency in microbial cultivation. These efforts were devoted to the attainment for diverse growth conditions, simulation of in situ conditions and achievement of high-throughput rates. As a result, prokaryotes catalysing novel redox reactions as well as representatives of abundant, but not-yet cultured taxa, were isolated. Significant insights into microbial physiology have been made by studying the small number of prokaryotes already cultured. However, despite these numerous breakthroughs, microbial cultivation is still a low-throughput process. The main hindrance to cultivation is likely due to the prevailing lack of knowledge on targeted species. In this review, we... |
| Tipo: Text |
Palavras-chave: High throughput cultivation; Cell cell communication; Microbial community; Metabolism; Isolation; Culture. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6820.pdf |
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| Aliabadi,Nasrin; Aminzadeh,Saeed; Karkhane,Ali Asghar; Haghbeen,Kamahldin. |
| Abstract Twelve bacterial strains isolated from shrimp farming ponds were screened for their growth activity on chitin as the sole carbon source. The highly chitinolytic bacterial strain was detected by qualitative cup plate assay and tentatively identified to be Cohnella sp. A01 based on 16S rDNA sequencing and by matching the key morphological, physiological, and biochemical characteristics. The cultivation of Cohnella sp. A01 in the suitable liquid medium resulted in the production of high levels of enzyme. The colloidal chitin, peptone, and K2HPO4 represented the best carbon, nitrogen, and phosphorus sources, respectively. Enzyme production by Cohnella sp. A01 was optimized by the Taguchi method. Our results demonstrated that inoculation amount and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biochemical characterization; Chitinase; Cohnella sp. A01; Isolation; Thermostable. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400931 |
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| Alves,Clebert José; Figueiredo,Salomão Moreira de; Azevedo,Sérgio Santos de; Clementino,Inácio José; Keid,Lara Borges; Vasconcellos,Sílvio Arruda; Batista,Carolina de Sousa Américo; Rocha,Vivianne Cambuí Mesquita; Higino,Severino Silvano. |
| To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucella ovis; Isolation; PCR; Ovine; Paraíba State. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200016 |
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| Barreto,Maria Lucia; Nascimento,Elmiro Rosendo do; Campos,Carlos Augusto de Martino; Nascimento,Maria da Graça Fichel do; Lignon,Gilberto Brasil; Lira,Marie Luce Flores; Silva,Ricardo G.. |
| This work was conducted on rats in two premises located in Niterói and Rio de Janeiro, Brazil. One is classified as conventional controlled and the other, conventional. The objective of the present study was to detect the presence of Mycoplasma pulmonis in animals with symptoms of respiratory disease and low reproductive performance. In the conventional controlled premises, 16 rats of Wistar-Furth strain were necropsied while in the conventional premises necropsy was performed on 12 rats of Hooded Lister strain. The clinical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturing while the sera were tested for antibody detection. From 28 rats, 57.14% (16/28) were culture positive for M. pulmonis, being 81.25%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma pulmonis; Diagnosis; Isolation; Serology; Rats. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300015 |
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| Cihan,Arzu Coleri; Tekin,Nilgun; Ozcan,Birgul; Cokmus,Cumhur. |
| Previously isolated 115 endospore-forming bacilli were basically grouped according to their temperature requirements for growth: the thermophiles (74%), the facultative thermophiles (14%) and the mesophiles (12%). These isolates were taken into 16S rRNA gene sequence analyses, and they were clustered among the 7 genera: Anoxybacillus, Aeribacillus, Bacillus, Brevibacillus, Geobacillus, Paenibacillus, and Thermoactinomycetes. Of these bacilli, only the thirty two isolates belonging to genera Bacillus (16), Brevibacillus (13), Paenibacillus (1) and Thermoactinomycetes (2) were selected and presented in this paper. The comparative sequence analyses revealed that the similarity values were ranged as 91.4-100 %, 91.8- 99.2 %, 92.6- 99.8 % and 90.7 - 99.8 %... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Isolation; Temperature requirement; Endospore-forming bacilli; 16S rRNA gene; ARDRA. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100037 |
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| Coêlho,Mariza M.; Ferreira-Nozawa,Monica S.; Nozawa,Sérgio R.; Santos,André L.W.. |
| Endophytic bacteria from three arboreal species native to the Amazon (Carapa guianenses, Ceiba pentandra, and Swietenia macrophylla), were isolated and identified, through partial sequencing of the 16S rRNA encoding gene. From these, 16 isolates were obtained, although, when compared to sequences deposited in GenBank, only seven had produced identifiable fragments. Bacillus, Pantoea and two non-culturable samples were identified. Results obtained through sequence analysis revealed low genetic diversity across the isolates, even when analyzing different species and plant structures. This is the first report concerning the isolation and identification of endophytic bacteria in these plant species. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Isolation; Sequencing; Bacillus; Pantoea. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400021 |
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| Costa,Diego Figueiredo da; Silva,Aline Ferreira da; Brasil,Arthur Willian de Lima; Loureiro,Ana Paula Pereira; Santos,Fabrine Alexandre dos; Azevedo,Sergio Santos de; Lilenbaum,Walter; Alves,Clebert José. |
| ABSTRACT: Sheep breeding is an economic activity of great value for the Brazilian livestock industry, and infectious diseases can significantly compromise its productivity. The aim of this study was to detect Leptospira spp. in mixed breed sheep from a semiarid region of the State of Paraíba, Northeast Brazil. In total, 49 blood and renal tissue samples, as well as 25 urine samples, were collected from 49 sheep in the slaughter line. The microscopic agglutination test (MAT) was used to verify the presence of anti -Leptospira spp. antibodies in blood samples. Renal tissue and urine samples were analyzed by both PCR and bacterial culture. Sero reactivity was observed in 8.2% (4/49) of animals. Three renal samples were positive for leptospiral DNA by PCR... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; Small ruminants; PCR; Serology; Isolation; Slaughterhouse. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000200451 |
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| Ding,Jiannan; Zhang,Ruiyong; Yu,Yizun; Jin,Decai; Liang,Changli; Yi,Yang; Zhu,Wei; Xia,Jinlan. |
| A novel thermoacidophilic iron and sulfur-oxidizing archaeon, strain YN25, was isolated from an in situ enriched acid hot spring sample collected in Yunnan, China. Cells were irregular cocci, about 0.9-1.02 µm×1.0-1.31 µm in the medium containing elemental sulfur and 1.5-2.22 µm×1.8-2.54 µm in ferrous sulfate medium. The ranges of growth and pH were 50-85 (optimum 65) and pH 1.0-6.0 (optimum 1.5-2.5). The acidophile was able to grow heterotrophically on several organic substrates, including various monosaccharides, alcohols and amino acids, though the growth on single substrate required yeast extract as growth factor. Growth occurred under aerobic conditions or via anaerobic respiration using elemental sulfur as terminal electron acceptor. Results of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Isolation; Identification; Acidianus manzaensis; Bioleaching. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200016 |
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| Domingues,S.F.S.; Ferreira,H.S.; Muniz,J.A.P.C.; Lima,A.K.F.; Ohashi,O.M.; Figueiredo,J.R.; Silva,L.D.M.. |
| The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Monkey; Preantral follicle; Isolation; Ovary; Cebus apella. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300009 |
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| Ferreira,Thais Sebastiana Porfida; Felizardo,Maria Roberta; Gobbi,Debora Dirani Sena de; Moreno,Marina; Moreno,Andrea Micke. |
| Cats are often described as carriers of Pasteurella multocida in their oral microbiota. This agent is thought to cause pneumonia, conjunctivitis, rhinitis, gingivostomatitis, abscess and osteonecrosis in cats. Human infection with P. multocida has been described in several cases affecting cat owners or after cat bites. In Brazil, the cat population is approximately 21 million animals and is increasing, but there are no studies of the presence of P. multocida in the feline population or of human cases of infection associated with cats. In this study, one hundred and ninety-one healthy cats from owners and shelters in São Paulo State, Brazil, were evaluated for the presence of P. multocida in their oral cavities. Twenty animals were positive for P.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cat; Pasteurela multocida; Virulence genes; Resistance; Isolation. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100271 |
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| Genaro,G.; Schmidek,W.R.; Franci,C.R.. |
| Studies of behavior, endocrinology and physiology have described experiments in which animals housed in groups or in isolation were normally tested individually. The isolation of the animal from its group for testing is perhaps the most common situation used today in experimental procedures, i.e., there is no consideration of the acute stress which occurs when the animal is submitted to a situation different from that it is normally accustomed to, i.e., group living. In the present study, we used 90 male 120-day-old rats (Rattus norvegicus) divided into 5 groups of 18 animals, which were housed 3 per cage, in a total of 6 cages. The animals were tested individually or with their groups for exploratory behavior. Hormones were determined by radioimmunoassay... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Isolation; Group; Stress; Reproduction; ACTH; Luteinizing hormone; Corticosterone; Prolactin. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600008 |
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| Ilhan,Ziya; Karaca,Mehmet; Ekin,Ismail Hakki; Solmaz,Hasan; Akkan,Hasan Altan; Tutuncu,Mehmet. |
| Abstract The Van cat is a domestic landrace found in the Van province of eastern Turkey. In this study, we aimed to determine the seasonal carriage of dermatophytes in Van cats without clinical lesions. A total of 264 hair specimens were collected from clinically healthy cats in and around the Van Province. Of these samples, 30.3% were obtained in spring, 30.6% in summer, 16.6% in autumn, and 22.3% in winter; 45.1% of samples were from male cats and the rest from female ones. Of the studied cats, 118 were younger than 1 year, 78 were 1–3 years old, and 68 were older than 3 years. The specimens were subjected to direct microscopic examination with 15% potassium hydroxide and cultured on Sabouraud dextrose agar and dermatophyte test medium supplemented with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carriage; Dermatophytes; Isolation; Van cat. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100225 |
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| Issa,Marina de Azevedo; Soares Filho,Paulo Martins; Fonseca Júnior,Antônio Augusto; Hodon,Mikael Arrais; Santos,Lílian Cristian dos; Reis,Jenner Karlisson Pimenta dos; Cerqueira Leite,Rômulo. |
| Abstract For the definitive diagnosis of bovine tuberculosis, isolation of the etiologic agent is required. However, there is no consensus on the best methodology for isolation of Mycobacterium bovis in Brazil. This study evaluated the most used decontaminants and culture media in the country, in order to identify the best combination for the Brazilian samples. Three decontaminants - 2% sodium hydroxide (w/v), 0.75% hexadecylpiridinium chloride (w/v) and 5% sulphuric acid (v/v) and four culture media - 7H11 Middlebrook with additives and OADC supplement “A” (7H11 A), the same media with another supplement trademark (7H11 B), tuberculosis blood agar (B83) and Stonebrink's medium were compared. Regarding the isolation, there were no significant differences... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium bovis; Diagnosis; Middlebrook 7H11; Isolation; Sulphuric acid. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100139 |
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| Registros recuperados: 56 | |
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